Calcification and silicification: fossilization potential of cyanobacteria from stromatolites of Niuafo'ou's Caldera Lakes (Tonga) and implications for the early fossil record

Calcification and silicification processes of cyanobacterial mats that form stromatolites in two caldera lakes of Niuafo'ou Island (Vai Lahi and Vai Si'i) were evaluated, and their importance as analogues for interpreting the early fossil record are discussed. It has been shown that the potential for morphological preservation of Niuafo'ou cyanobacteria is highly dependent on the timing and type of mineral phase involved in the fossilization process. Four main modes of mineralization of cyanobacteria organic parts have been recognized: (i) primary early postmortem calcification by aragonite nanograins that transform quickly into larger needle-like crystals and almost totally destroy the cellular structures, (ii) primary early postmortem silicification of almost intact cyanobacterial cells that leave a record of spectacularly well-preserved cellular structures, (iii) replacement by silica of primary aragonite that has already recrystallized and obliterated the cellular structures, (iv) occasional replacement of primary aragonite precipitated in the mucopolysaccharide sheaths and extracellular polymeric substances by Al-Mg-Fe silicates. These observations suggest that the extremely scarce earliest fossil record may, in part, be the result of (a) secondary replacement by silica of primary carbonate minerals (aragonite, calcite, siderite), which, due to recrystallization, had already annihilated the cellular morphology of the mineralized microbiota or (b) relatively late primary silicification of already highly degraded and no longer morphologically identifiable microbial remains.     

Abundance and diversity of microbial inhabitants in European spacecraft-associated clean rooms

The determination of the microbial load of a spacecraft en route to interesting extraterrestrial environments is mandatory and currently based on the culturable, heat-shock-surviving portion of microbial contaminants. Our study compared these classical bioburden measurements as required by NASA's and ESA's guidelines for the microbial examination of flight hardware, with molecular analysis methods (16S rRNA gene cloning and quantitative PCR) to further develop our understanding of the diversity and abundance of the microbial communities of spacecraft-associated clean rooms. Three samplings of the Herschel Space Observatory and its surrounding clean rooms were performed in two different European facilities. Molecular analyses detected a broad diversity of microbes typically found in the human microbiome with three bacterial genera (Staphylococcus, Propionibacterium, and Brevundimonas) common to all three locations. Bioburden measurements revealed a low, but heterogeneous, abundance of spore-forming and other heat-resistant microorganisms. Total cell numbers estimated by quantitative real-time PCR were typically 3 orders of magnitude greater than those determined by viable counts, which indicates a tendency for traditional methods to underestimate the extent of clean room bioburden. Furthermore, the molecular methods allowed the detection of a much broader diversity than traditional culture-based methods.     

The PROCESS experiment: an astrochemistry laboratory for solid and gaseous organic samples in low-earth orbit

The PROCESS (PRebiotic Organic ChEmistry on the Space Station) experiment was part of the EXPOSE-E payload outside the European Columbus module of the International Space Station from February 2008 to August 2009. During this interval, organic samples were exposed to space conditions to simulate their evolution in various astrophysical environments. The samples used represent organic species related to the evolution of organic matter on the small bodies of the Solar System (carbonaceous asteroids and comets), the photolysis of methane in the atmosphere of Titan, and the search for organic matter at the surface of Mars. This paper describes the hardware developed for this experiment as well as the results for the glycine solid-phase samples and the gas-phase samples that were used with regard to the atmosphere of Titan. Lessons learned from this experiment are also presented for future low-Earth orbit astrochemistry investigations.     

LIFE: Life Investigation For Enceladus A Sample Return Mission Concept in Search for Evidence of Life

Abstract Life Investigation For Enceladus (LIFE) presents a low-cost sample return mission to Enceladus, a body with high astrobiological potential. There is ample evidence that liquid water exists under ice coverage in the form of active geysers in the "tiger stripes" area of the southern Enceladus hemisphere. This active plume consists of gas and ice particles and enables the sampling of fresh materials from the interior that may originate from a liquid water source. The particles consist mostly of water ice and are 1-10?μ in diameter. The plume composition shows H(2)O, CO(2), CH(4), NH(3), Ar, and evidence that more complex organic species might be present. Since life on Earth exists whenever liquid water, organics, and energy coexist, understanding the chemical components of the emanating ice particles could indicate whether life is potentially present on Enceladus. The icy worlds of the outer planets are testing grounds for some of the theories for the origin of life on Earth. The LIFE mission concept is envisioned in two parts: first, to orbit Saturn (in order to achieve lower sampling speeds, approaching 2 km/s, and thus enable a softer sample collection impact than Stardust, and to make possible multiple flybys of Enceladus); second, to sample Enceladus' plume, the E ring of Saturn, and the Titan upper atmosphere. With new findings from these samples, NASA could provide detailed chemical and isotopic and, potentially, biological compositional context of the plume. Since the duration of the Enceladus plume is unpredictable, it is imperative that these samples are captured at the earliest flight opportunity. If LIFE is launched before 2019, it could take advantage of a Jupiter gravity assist, which would thus reduce mission lifetimes and launch vehicle costs. The LIFE concept offers science returns comparable to those of a Flagship mission but at the measurably lower sample return costs of a Discovery-class mission. Key Words: Astrobiology-Habitability-Enceladus-Biosignatures. Astrobiology 12, 730-742.     

A new spectral index to detect Poaceae grass abundance in Mongolian grasslands

The objectives of the present study were to develop a new index based on remotely-sensed data for detecting the abundance of grasses in the family Poaceae, which has a high palatability for livestock in Mongolia, and to map the distribution of these grasses in the semi-arid Mongolian steppes. We measured ground-based spectral reflectance of pure plant leaves – including Poaceae grasses – and soils, as well as in-situ in the Mongolian grasslands. The hyper-spectral data, taken by a spectroradiometer, were converted into four multi-spectral bands (i.e., blue, green, red, and NIR) to simulate satellite-based imagery data. In order to magnify the characteristics of the spectral signal of Poaceae, NGBDI (Normalized Green-Blue Difference Index), NGRDI (Normalized Green-Red Difference Index), NDVI (Normalized Difference Vegetation Index), NNBDI (Normalized NIR-Blue Difference Index) were calculated from the four multi-spectral reflectance values. Poaceae Abundance Index (PAI) was derived by combining these four normalized difference indices. PAI was found out to be a good indicator to discriminate Poaceae grass from the other plant spectral data.     

Day-to-day variability of equatorial anomaly in GPS-TEC during low solar activity period

The ionospheric total electron content (TEC) in the northern hemispheric equatorial ionization anomaly (EIA) crest region is investigated by using dual-frequency signals of the Global Positioning System (GPS) acquired from Rajkot (Geog. Lat. 22.29°N, Geog. Long. 70.74°E; Geom. Lat. 14.21°N, Geom. Long. 144.90°E), India. The day-to-day variability of EIA characteristics is examined during low solar activity period (F10.7∼83 sfu). It is found that the daily maximum TEC at EIA crest exhibits a day-to-day and strong semi-annual variability. The seasonal anomaly and equinoctial asymmetry in TEC at EIA is found non-existent and weaker, respectively. We found a moderate and positive correlation of daily magnitude of crest, Ic with daily F10.7 and EUV fluxes with a correlation coefficient of 0.43 and 0.33, respectively indicating an existence of a short-term relation between TEC at EIA and the solar radiation even during low solar activity period. The correlation of daily Ic with Dst index is also moderate (r = −0.35), whereas no correlation is found with the daily Kp index (r = 0.14) respectively. We found that the magnitude of EIA crest is moderately correlated with solar flux in all seasons except winter where it is weakly related (0.27). The magnitude of EIA crest is also found highly related with EEJ strength in spring (r = 0.69) and summer (r = 0.65) than autumn (0.5) and winter (r = 0.47), though EEJ is stronger in autumn than spring.     

Effects of solar modulation on the cosmic ray positron fraction

We implemented a 2D Monte Carlo model to simulate the solar modulation of galactic cosmic rays. The model is based on the Parker’s transport equation which contains diffusion, convection, particle drift and energy loss. Following the evolution in time of the solar activity, we are able to modulate a local interstellar spectrum (LIS), that we assumed isotropic beyond the termination shock, down to the Earth position inside the heliosphere. In this work we focused our attention to the cosmic ray positron fraction at energy below ∼10 GeV, showing how the particle drift processes could explain different results for AMS-01 and PAMELA. We compare our modulated spectra with observations at Earth, and then make a prediction of the cosmic ray positron fraction for the AMS-02 experiment.     

Comparison of Antarctic riometer radio wave absorption and THEMIS mission energetic electron fluxes

Simultaneous observations of in situ plasma properties in the tail of the Earth’s magnetosphere and of ground based instruments, lying on the same geomagnetic field lines, have recently proved to yield significant new results. In most cases magnetosphere ionosphere interactions during the night-time northern hemisphere conditions are studied. Here, observations of energetic electrons in the tail of the Earth’s magnetosphere made by the THEMIS mission satellites are compared with auroral radio wave absorption determined by riometers in the Antarctic for sunlit conditions. Days for which satellites and riometers are connected by the same geomagnetic field line are selected using a geomagnetic field model. The six days analysed show clear associations between fluxes and absorptions in some cases. However, these do not necessarily correspond to conjugacy intervals. Hours of positive associations are 1.65 times those for negative associations, all hours and days considered (1.42–3.6 on five days and 0.58 on the other day). These computations are assumed appropriate since the footprints of the satellites used approximately follow corrected geomagnetic parallels for all six days studied. The use of a finer parameterization of geomagnetic models to determine conjugacy may be needed.     

GNSS-R concept extended by a fine orbit tuning

Small changes in semimajor axis of the orbits selected for the GNSS-R [R as Reflectometry] satellites, so-called fine orbit tuning, known from the ESA’s Gravity and steady-state Ocean Circulation Explorer mission, can dramatically increase the number of nadir and off-nadir reflecting points and, in turn, can enhance the capability of the concept of bistatic altimetry (GNSS Reflectometry) without additional costs. The application of our suggestion is feasible for a satellite which will be equipped by thrusters for the orbit keeping. During the mission lifetime several orbit tunings are feasible, just to transfer from one to another orbit. Then we can study short-periodic or longer-periodic features, according to scientific goals defined for the mission. The shortest cycles (few days), corresponding to the required revisit time (defined by ESA), may be subcycles of much longer cycles (repeat periods).     

Ionospheric response of equatorial and low latitude F-region during the intense geomagnetic storm on 24–25 August 2005

In this investigation, we present and discuss the response of the ionospheric F-region in the South American and East Asian sectors during an intense geomagnetic storm in August 2005. The geomagnetic storm studied reached a minimum Dst of −216 nT at 12:00 UT on 24 August. In this work ionospheric sounding data obtained of 24, 25, and 26 August 2005 at Palmas (PAL; 10.2° S, 48.2° W; dip latitude 6.6° S), São José dos Campos (SJC, 23.2° S, 45.9° W; dip latitude 17.6° S), Brazil, Ho Chi Minh City, (HCM; 10.5° N, 106.3° E; dip latitude 2.9° N), Vietnam, Okinawa (OKI; 26.3° N, 127.8° E; dip latitude 21.2° N), Japan, are presented. Also, the GPS observations obtained at different stations in the equatorial and low-latitude regions in the Brazilian sector are presented. On the night of 24–25 August 2005, the h′F variations show traveling ionospheric disturbances associated with Joule heating in the auroral zone from SJC to PAL. The foF2 variations show a positive storm phase on the night of 24–25 August at PAL and SJC during the recovery phase. Also, the GPS-VTEC observations at several stations in the Brazilian sector show a fairly similar positive storm phase on 24 August. During the fast decrease of Dst (between 10:00 and 11:00 UT) on 24 August, there is a prompt penetration of electric field of magnetospheric origin that result in abrupt increase (∼12:00 UT) in foF2 at PAL, SJC (Brazil) and OKI (Japan) and in VTEC at IMPZ, BOMJ, PARA and SMAR (Brazil). OKI showed strong oscillations of the F-region on the night 24 August resulted to the propagation of traveling atmospheric disturbances (TADs) by Joule heating in the auroral region. These effects result a strong positive observed at OKI station. During the daytime on 25 August, in the recovery phase, the foF2 observations showed positive ionospheric storm at HCM station. Some differences in the latitudinal response of the F-region is also observed in the South American and East Asian sectors.